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Fig. 3 | Plant Methods

Fig. 3

From: A CRISPR/dCas9 toolkit for functional analysis of maize genes

Fig. 3

Target gene expression analysis in maize protoplasts transfected with CRISPRa and CRISPRi constructs. Maize protoplasts were co-transfected with pDA2 (a) or pDA4 (b) with individual ChlH gRNA candidates, or with pDA3 (c) and individual TrxH gRNA candidates. To test combinations of multiplexed PDS1 gRNAs separated by tRNAs, maize protoplasts were co-transfected with pDA4 (d) or pDA3 (e). The ability of the gRNAs to repress (pDA2 and pDA4) or activate (pDA3) the target genes was assessed by qRT-PCR. Data represent triplicate samples from one biological replicate, where asterisks (*) indicate significant differences from the negative control, assessed by Student’s t-test where p < 0.1. Error bars are standard deviation

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