Fig. 6
Total wax amount per unit area of isolated and washed adaxial and abaxial cuticles of A. thaliana. Total wax amount was calculated as the sum of C16–C30 acids, C24–C30 alcohols and C20–C33 alkanes (a). Total wax amount after omission of C16-C18 acids (b). Mean values from 5 replicates are shown. On average, wax from 20 leaves from 12 plants of Col-0 ecotype was collected in one replicate to make up 20 cm2 of cuticle area. Bars represent one standard deviation. Symbols: ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001