Fig. 5
Wax amount per unit area of extracted isolated cuticles and washed leaf surfaces of A. thaliana Col-0 wild type. C16–C30 acids, C24–C30 alcohols and C20–C33 alkanes were evaluated separately from adaxial (a) and abaxial (b) cuticles/leaf surfaces. Values for individual compounds on both leaf sides are also shown (c). Mean values from five replicates are shown. On average, wax from 20 leaves from 12 plants was pooled in any one replicate to make up 20 cm2 of cuticle area. Bars represent one standard deviation. Symbols: ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001