Fig. 3

Scanning electron microscopy (a–f) and cryomicroscopy (g–h) of isolated cuticles of Arabidopsis thaliana Col-0 wild type. Adaxial external (a) and internal (b), abaxial external (c) and internal (d) sides of cuticles observed in secondary electron mode are shown. Details of the relief of abaxial cuticle and a stoma seen at higher magnification from the external (e) and internal (f) side, respectively, are included to show that a cuticle was present in substomatal cavities (f). Adaxial cuticles observed by cryomicroscopy are shown (g, h). Outlines of stomatal guard cells and pores (s), trichome envelopes (t), and borders of formerly present epidermal cells (arrowheads) are visible