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Table 2 Method validation

From: An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS

Compound Determined spiked content (pmol)a Method precision (%RSD)a Method accuracy (%bias)a Determined spiked content (pmol)b Method precision (%RSD)b Method accuracy (%bias)b
Root exudates (20 ml)
 Strigol 1.03 ± 0.03 3.0 3.45 9.71 ± 0.20 2.0 − 2.94
 Solanacol 0.95 ± 0.03 2.7 − 4.90 8.78 ± 0.12 1.3 − 12.21
 Orobanchol 0.93 ± 0.05 4.9 − 6.89 9.42 ± 0.10 1.0 − 5.78
 Sorgomol 1.03 ± 0.05 5.3 2.83 9.28 ± 0.14 1.5 − 7.23
 Sorgolactone 1.03 ± 0.05 5.3 2.83 9.28 ± 0.14 1.5 − 7.23
 4-DO 1.06 ± 0.04 3.7 5.89 9.57 ± 0.24 2.5 − 4.32
 5-DS 1.03 ± 0.04 3.6 2.79 9.39 ± 0.15 1.6 − 6.08
Root tissue (150 mg)
 Strigol 1.07 ± 0.05 4.4 0.67 9.81 ± 0.12 1.2 − 1.91
 Solanacol 1.05 ± 0.04 3.4 0.47 8.72 ± 0.09 1.0 − 12.83
 Orobanchol 1.09 ± 0.04 3.8 0.92 9.72 ± 0.25 2.6 − 2.80
 Sorgomol 1.13 ± 0.14 12.3 1.33 10.12 ± 0.04 0.4 1.18
 Sorgolactone 1.07 ± 0.04 3.3 0.66 9.70 ± 0.15 1.6 − 2.95
 4-DO 1.08 ± 0.01 1.2 0.81 9.78 ± 0.09 0.9 − 2.15
 5-DS 1.05 ± 0.01 0.9 0.46 10.20 ± 0.13 1.2 2.05
  1. The precision and accuracy evaluated by spiking a fixed amount of sorghum root exudate (20 ml) and root tissue (150 mg of fresh weight) with analytes at two different concentrations (a 1 pmol, and b 10 pmol) prior to individual extraction procedures. Values are mean ± SD (n = 4)