Table 2 Method validation
From: An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS
Compound | Determined spiked content (pmol)a | Method precision (%RSD)a | Method accuracy (%bias)a | Determined spiked content (pmol)b | Method precision (%RSD)b | Method accuracy (%bias)b |
|---|---|---|---|---|---|---|
Root exudates (20 ml) | ||||||
Strigol | 1.03 ± 0.03 | 3.0 | 3.45 | 9.71 ± 0.20 | 2.0 | − 2.94 |
Solanacol | 0.95 ± 0.03 | 2.7 | − 4.90 | 8.78 ± 0.12 | 1.3 | − 12.21 |
Orobanchol | 0.93 ± 0.05 | 4.9 | − 6.89 | 9.42 ± 0.10 | 1.0 | − 5.78 |
Sorgomol | 1.03 ± 0.05 | 5.3 | 2.83 | 9.28 ± 0.14 | 1.5 | − 7.23 |
Sorgolactone | 1.03 ± 0.05 | 5.3 | 2.83 | 9.28 ± 0.14 | 1.5 | − 7.23 |
4-DO | 1.06 ± 0.04 | 3.7 | 5.89 | 9.57 ± 0.24 | 2.5 | − 4.32 |
5-DS | 1.03 ± 0.04 | 3.6 | 2.79 | 9.39 ± 0.15 | 1.6 | − 6.08 |
Root tissue (150 mg) | ||||||
Strigol | 1.07 ± 0.05 | 4.4 | 0.67 | 9.81 ± 0.12 | 1.2 | − 1.91 |
Solanacol | 1.05 ± 0.04 | 3.4 | 0.47 | 8.72 ± 0.09 | 1.0 | − 12.83 |
Orobanchol | 1.09 ± 0.04 | 3.8 | 0.92 | 9.72 ± 0.25 | 2.6 | − 2.80 |
Sorgomol | 1.13 ± 0.14 | 12.3 | 1.33 | 10.12 ± 0.04 | 0.4 | 1.18 |
Sorgolactone | 1.07 ± 0.04 | 3.3 | 0.66 | 9.70 ± 0.15 | 1.6 | − 2.95 |
4-DO | 1.08 ± 0.01 | 1.2 | 0.81 | 9.78 ± 0.09 | 0.9 | − 2.15 |
5-DS | 1.05 ± 0.01 | 0.9 | 0.46 | 10.20 ± 0.13 | 1.2 | 2.05 |