|
Authentic compound
|
Retention timea (min)
|
Scan mode
|
Precursor ion
|
Product ion I
|
Product ion II
|
Cone voltage (V)
|
Collision energy (eV)
|
LODb (fmol)
|
Linear range (pmol)
|
R2
|
|---|
|
Strigol
|
3.50 ± 0.02
|
+
|
347
|
96.9
|
233
|
18
|
15
|
2.5
|
0.0125–2.5
|
0.995
|
|
Solanacol
|
3.54 ± 0.01
|
+
|
343
|
96.9
|
183
|
15
|
20
|
0.25
|
0.00125–2.5
|
0.997
|
|
Orobanchol
|
4.59 ± 0.01
|
+
|
347
|
97
|
205
|
15
|
20
|
1.25
|
0.0025–2.5
|
0.998
|
|
Sorgomol
|
4.85 ± 0.02
|
+
|
347
|
233
|
133
|
15
|
25
|
2.5
|
0.0125–2.5
|
0.992
|
|
Sorgolactone
|
7.48 ± 0.01
|
+
|
317.1
|
96.9
|
133
|
20
|
23
|
0.125
|
0.00025–2.5
|
0.998
|
|
4-DO
|
7.94 ± 0.01
|
+
|
331.1
|
96.9
|
216
|
15
|
18
|
0.125
|
0.00025–2.5
|
0.999
|
|
5-DS
|
8.00 ± 0.01
|
+
|
331.1
|
96.9
|
216
|
15
|
18
|
0.125
|
0.00025–2.5
|
0.999
|
|
Carlactonoic acid
|
8.28 ± 0.01
|
–
|
331
|
113
|
242
|
20
|
12
|
2.5
|
0.005–2.5
|
0.997
|
|
Carlactone
|
9.63 ± 0.02
|
+
|
303
|
96.9
|
285
|
15
|
18
|
2.5
|
0.0125–2.5
|
0.992
|
|
Internal standard
| | | | | | | |
Quantified analyte
|
|---|
|
GR24
|
5.13 ± 0.01
|
+
|
299
|
96.9
|
185
|
20
|
20
|
Strigol, solanacol, orobanchol, sorgomol
|
|
[2H6]-2′-epi-5-DS
|
7.88 ± 0.01
|
+
|
337
|
96.9
|
221
|
15
|
18
|
4-DO
|
|
[2H6]-5-DS
|
7.94 ± 0.01
|
+
|
337
|
96.9
|
221
|
15
|
18
|
Sorgolactone, 5-DS
|
- aValues are mean ± SD (n = 5); b The signal-to-noise ratio was set to 3:1
- MRM transitions of authentic SLs and corresponding internal standards, including optimized instrument settings and UHPLC–MS/MS method parameters (retention time, limit of detection—LOD, linear range and coefficient of determination—r2)
