An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS

Table 1 Method parameters

Authentic compound	Retention time^a (min)	Scan mode	Precursor ion	Product ion I	Product ion II	Cone voltage (V)	Collision energy (eV)	LOD^b (fmol)	Linear range (pmol)	R²
Strigol	3.50 ± 0.02	+	347	96.9	233	18	15	2.5	0.0125–2.5	0.995
Solanacol	3.54 ± 0.01	+	343	96.9	183	15	20	0.25	0.00125–2.5	0.997
Orobanchol	4.59 ± 0.01	+	347	97	205	15	20	1.25	0.0025–2.5	0.998
Sorgomol	4.85 ± 0.02	+	347	233	133	15	25	2.5	0.0125–2.5	0.992
Sorgolactone	7.48 ± 0.01	+	317.1	96.9	133	20	23	0.125	0.00025–2.5	0.998
4-DO	7.94 ± 0.01	+	331.1	96.9	216	15	18	0.125	0.00025–2.5	0.999
5-DS	8.00 ± 0.01	+	331.1	96.9	216	15	18	0.125	0.00025–2.5	0.999
Carlactonoic acid	8.28 ± 0.01	–	331	113	242	20	12	2.5	0.005–2.5	0.997
Carlactone	9.63 ± 0.02	+	303	96.9	285	15	18	2.5	0.0125–2.5	0.992

Internal standard								Quantified analyte
GR24	5.13 ± 0.01	+	299	96.9	185	20	20	Strigol, solanacol, orobanchol, sorgomol
[²H₆]-2′-epi-5-DS	7.88 ± 0.01	+	337	96.9	221	15	18	4-DO
[²H₆]-5-DS	7.94 ± 0.01	+	337	96.9	221	15	18	Sorgolactone, 5-DS

^aValues are mean ± SD (n = 5); ^b The signal-to-noise ratio was set to 3:1
MRM transitions of authentic SLs and corresponding internal standards, including optimized instrument settings and UHPLC–MS/MS method parameters (retention time, limit of detection—LOD, linear range and coefficient of determination—r²)

ISSN: 1746-4811