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Table 1 Method parameters

From: An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS

Authentic compound Retention timea (min) Scan mode Precursor ion Product ion I Product ion II Cone voltage (V) Collision energy (eV) LODb (fmol) Linear range (pmol) R2
Strigol 3.50 ± 0.02 + 347 96.9 233 18 15 2.5 0.0125–2.5 0.995
Solanacol 3.54 ± 0.01 + 343 96.9 183 15 20 0.25 0.00125–2.5 0.997
Orobanchol 4.59 ± 0.01 + 347 97 205 15 20 1.25 0.0025–2.5 0.998
Sorgomol 4.85 ± 0.02 + 347 233 133 15 25 2.5 0.0125–2.5 0.992
Sorgolactone 7.48 ± 0.01 + 317.1 96.9 133 20 23 0.125 0.00025–2.5 0.998
4-DO 7.94 ± 0.01 + 331.1 96.9 216 15 18 0.125 0.00025–2.5 0.999
5-DS 8.00 ± 0.01 + 331.1 96.9 216 15 18 0.125 0.00025–2.5 0.999
Carlactonoic acid 8.28 ± 0.01 331 113 242 20 12 2.5 0.005–2.5 0.997
Carlactone 9.63 ± 0.02 + 303 96.9 285 15 18 2.5 0.0125–2.5 0.992
Internal standard         Quantified analyte
GR24 5.13 ± 0.01 + 299 96.9 185 20 20 Strigol, solanacol, orobanchol, sorgomol
[2H6]-2′-epi-5-DS 7.88 ± 0.01 + 337 96.9 221 15 18 4-DO
[2H6]-5-DS 7.94 ± 0.01 + 337 96.9 221 15 18 Sorgolactone, 5-DS
  1. aValues are mean ± SD (n = 5); b The signal-to-noise ratio was set to 3:1
  2. MRM transitions of authentic SLs and corresponding internal standards, including optimized instrument settings and UHPLC–MS/MS method parameters (retention time, limit of detection—LOD, linear range and coefficient of determination—r2)