Fig. 3
From: An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS
Chromatographic separation of SLs and biosynthetic precursors analyzed by UHPLC–MS/MS. The figure shows normalized MRM chromatograms of strigol (1), solanacol (2), orobanchol (3), sorgomol (4), GR24 (5), sorgolactone (6), 4-deoxyorobanchol (4-DO, 7), 5-deoxystrigol (5-DS, 8), carlactonoic acid (9) and carlactone (10), represented by 0.5 pmol of each analyte injected onto Acquity UPLC® BEH C18 2.1 × 100 mm, 1.7 µm column and separated by gradient elution using 15 mM formic acid in both water and acetonitrile component of mobile phase