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Fig. 8 | Plant Methods

Fig. 8

From: An improved pollen number counting method using a cell counter and mesh columns

Fig. 8

Artificial pollen release and detection by the cell counter. Microscope observations of pollen grains from ‘Iwafune-9’ clone in CASYton at 0 min (a) and 30 min (b). Eighteen of the 22 pollen grains had released the pollen cell from the exine structure after 30 min. Bars = 50 μm. Arrow: released pollen cell, arrowhead: exine structure, asterisk: unreleased pollen. c Particle distributions at 0 min (magenta) and 30 min (green) for the same strobilus sample. Additional peaks derived from removed exine were observed in the sample after 30 min

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