TY - JOUR AU - Kakui, Hiroyuki AU - Tsurisaki, Eriko AU - Sassa, Hidenori AU - Moriguchi, Yoshinari PY - 2020 DA - 2020/09/14 TI - An improved pollen number counting method using a cell counter and mesh columns JO - Plant Methods SP - 124 VL - 16 IS - 1 AB - The determination of pollen number is important in evolutionary, agricultural, and medical studies. Tree species of the Cupressaceae family cause serious pollinosis worldwide. Although Japanese cedar (Cryptomeria japonica) is the most important forestry species in Japan, it is also the biggest cause of pollinosis in the country. Japanese cedar trees have been selected for growth speed and superior morphological traits and then cloned. These clones may vary in their pollen production, but there has been little research on how many pollen grains are produced by a single male strobilus (flower). A recently reported method for counting pollen number with a cell counter was applicable to Arabidopsis species and wheat, but was not suitable for Japanese cedar because the strobilus does not open with heating (e.g. 60 °C, overnight). SN - 1746-4811 UR - https://doi.org/10.1186/s13007-020-00668-4 DO - 10.1186/s13007-020-00668-4 ID - Kakui2020 ER -