Preparation
|
Chloroplast Yields (µg Chl/g FW)
|
Stromal Protein Yields (µg stromal protein/g FW)
|
Fold change (relative to tomato leaf #1)
|
References
|
---|
Tomato leaf #1a
|
5.61
|
1.56
|
1
|
This paper
|
Tomato leaf #2b
|
19.15
|
5.3
|
3.4
|
This paper
|
Tomato leaf #3c
|
26.50
|
7.95
|
4.7
|
This paper
|
Tomato green fruit plastids
|
2.7
|
–
|
0.5
|
Suzuki et al. [51]
|
Arabidopsis protoplasts
|
50–100
|
–
|
8.9–17.8
|
Fitzpatrick and Keegstra [59]
|
Arabidopsis leaves
|
5
|
–
|
1.1
|
Fitzpatrick and Keegstra [59]
|
Arabidopsis leaves
|
–
|
0.1–0.2
| |
Hall et al. [46]
|
Spinach leaves
|
4–5
|
–
|
0.7–1.1
|
Rensick et al. [60]
|
- aThe tomato leaf #1 chloroplast prep was performed prior to optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender
- bThe tomato leaf #2 chloroplast prep was performed with optimized blender time and speeds. Leaf segments were added in two batches into the blender
- cThe tomato leaf #3 chloroplast prep was performed with optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender