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Table 1 Chloroplast and stromal protein yields

From: Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins

Preparation

Chloroplast Yields (µg Chl/g FW)

Stromal Protein Yields (µg stromal protein/g FW)

Fold change (relative to tomato leaf #1)

References

Tomato leaf #1a

5.61

1.56

1

This paper

Tomato leaf #2b

19.15

5.3

3.4

This paper

Tomato leaf #3c

26.50

7.95

4.7

This paper

Tomato green fruit plastids

2.7

–

0.5

Suzuki et al. [51]

Arabidopsis protoplasts

50–100

–

8.9–17.8

Fitzpatrick and Keegstra [59]

Arabidopsis leaves

5

–

1.1

Fitzpatrick and Keegstra [59]

Arabidopsis leaves

–

0.1–0.2

 

Hall et al. [46]

Spinach leaves

4–5

–

0.7–1.1

Rensick et al. [60]

  1. aThe tomato leaf #1 chloroplast prep was performed prior to optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender
  2. bThe tomato leaf #2 chloroplast prep was performed with optimized blender time and speeds. Leaf segments were added in two batches into the blender
  3. cThe tomato leaf #3 chloroplast prep was performed with optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender