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Table 1 Chloroplast and stromal protein yields

From: Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins

Preparation Chloroplast Yields (µg Chl/g FW) Stromal Protein Yields (µg stromal protein/g FW) Fold change (relative to tomato leaf #1) References
Tomato leaf #1a 5.61 1.56 1 This paper
Tomato leaf #2b 19.15 5.3 3.4 This paper
Tomato leaf #3c 26.50 7.95 4.7 This paper
Tomato green fruit plastids 2.7 0.5 Suzuki et al. [51]
Arabidopsis protoplasts 50–100 8.9–17.8 Fitzpatrick and Keegstra [59]
Arabidopsis leaves 5 1.1 Fitzpatrick and Keegstra [59]
Arabidopsis leaves 0.1–0.2   Hall et al. [46]
Spinach leaves 4–5 0.7–1.1 Rensick et al. [60]
  1. aThe tomato leaf #1 chloroplast prep was performed prior to optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender
  2. bThe tomato leaf #2 chloroplast prep was performed with optimized blender time and speeds. Leaf segments were added in two batches into the blender
  3. cThe tomato leaf #3 chloroplast prep was performed with optimization of blender time and speed and ice slurry consistency. Leaf segments were added in two batches into the blender