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Fig. 6 | Plant Methods

Fig. 6

From: Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta

Fig. 6

Marker enzyme assays of different fractions during mitochondria isolation. Enzyme activity of plastidic, cytosolic and mitochondrial marker enzymes measured in the following fractions: Input (plant filtrate before incubation with SpyCatcher beads), Supernatant (plant filtrate after incubation with SpyCatcher beads, W1–W3 (Washing fractions) and beads (beads with isolated organelles). a Shows specific NADP-dependent glyceraldehyde-3-phosphate dehydrogenase activity (NADP: GAP-DH; plastidic marker), b shows specific alcohol dehydrogenase activity (ADH; cytosolic marker), c shows specific NADP-dependent glucose-6-phosphate dehydrogenase activity (cytosolic marker) and d shows specific fumarase activity as a marker for mitochondria enrichment

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