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Fig. 3 | Plant Methods

Fig. 3

From: Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta

Fig. 3

Validation of the subcellular localization by covalent interaction in planta between co-expressed organelle-specific SpyTag and eGFP-SpyCatcher. Organelle-specific SpyTag constructs and eGFP-SpyCatcher were transiently co-expressed in leaves of Nicotiana benthamiana plants. CLSM analysis was performed 72 h/96 h after infiltration. Expression of eGFP-SpyCatcher alone revealed cytosolic and nuclear localization (ad). When co-expressed with organelle-specific SpyTag constructs, eGFP fluorescence is directed towards the targeted organelles. Chloroplasts were targeted by co-expressing Plastid-SpyTag (eh), and mitochondria by co-expressing Mito-SpyTag (eh). Autofluorescence of chloroplasts is shown in blue (ah), the mitochondria marker IVD-mcherry is shown in red (il). Scale bars represent 10 µm

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