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Fig. 2 | Plant Methods

Fig. 2

From: Methods of analysis of chloroplast genomes of C3, Kranz type C4 and Single Cell C4 photosynthetic members of Chenopodiaceae

Fig. 2

PCR amplicons flanking a 4.8 kb insertion in the chloroplast genome of H. ammodendron (2–6) and H. persicum (8–12). Agarose gel electrophoresis of PCR products (a); diagram representing location and length of each amplicon (b). Expected amplicon sizes are 6607 (2 and 8), 7172 (3 and 9), 8132 (4 and 6), 3810 (5 and 11), and 4458 nt (6 and 12). Primers for PCRs 2–4 and 8–10 flank the ycf1 and ndhF genes. Primers for PCRs 5 and 11 flank flank the middle section of the 4.8 kb insertion and the ndhF gene. Primers for PCRs 6 and 12 flank the ycf1 gene and the middle section of the 4.8 kb insertion. 1 and 7: exACTGene DNA Ladders 1 kb DNA Ladder

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