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Table 5 Off-target enrichment statistics of Oxford Nanopore Technology sequencing reads against the apple genome

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

Base-caller

Chromosome

Start (bp)

End (bp)

Total size (bp)

Maximum coverage

Annotation

Guppy

Chr00

48263504

48264621

1117

28

within MD00G1202200

Guppy

Chr14a

30289152

30291917

2765

26

2,351 bp upstream of MD14G1221200

Albacore2

Chr14a

30289890

30291921

2031

26

 

Guppy

Chr15

47766093

47766888

795

26

within MD15G1386400

Guppy

Chr16

38910980

38919476

8496

41

spanning two genes: MD16G1282900 & MD16G1283000

  1. Off-targets sites presented had mapping quality equal or higher than 40 × and coverage equal or higher than 25 × .
  2. bp: base pairs
  3. aBoth chromosome 14 regions are the same. The apple reference genome (‘Golden Delicious’ double haploid GDDH13v1.1) [34] was used as reference.