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Table 5 Off-target enrichment statistics of Oxford Nanopore Technology sequencing reads against the apple genome

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

Base-caller Chromosome Start (bp) End (bp) Total size (bp) Maximum coverage Annotation
Guppy Chr00 48263504 48264621 1117 28 within MD00G1202200
Guppy Chr14a 30289152 30291917 2765 26 2,351 bp upstream of MD14G1221200
Albacore2 Chr14a 30289890 30291921 2031 26  
Guppy Chr15 47766093 47766888 795 26 within MD15G1386400
Guppy Chr16 38910980 38919476 8496 41 spanning two genes: MD16G1282900 & MD16G1283000
  1. Off-targets sites presented had mapping quality equal or higher than 40 × and coverage equal or higher than 25 × .
  2. bp: base pairs
  3. aBoth chromosome 14 regions are the same. The apple reference genome (‘Golden Delicious’ double haploid GDDH13v1.1) [34] was used as reference.