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Table 3 Oxford Nanopore Technology sequencing reads base-called using Albacore2 and Guppy

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

 

Base-called reads

Bases

Median read length

N50

Median read quality

All

Pass

All

Pass

All

Pass

All

Pass

All

Pass

Albacore2

7056

6082

112578716

106589486

9783.5

11359

30416

30910

9.11

9.28

Guppy

7056

6377

113972292

109479250

9891.5

11008

30635

30825

11.22

11.39

  1. Pass reads are those with a mean quality of 7 or more. Software versions: Albacore (v2.3.4, ONT) and Guppy (v3.2.4, ONT).