Skip to main content

Table 1 CRISPR RNA oligonucleotide sequences used for cleavage of genomic DNA in the apple MYB10 locus

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

Name

Sequence 5′-3′

Off-target score (%)

On-target activity score (%)

Chr

Start position

End position

crRNA_RF_1_F

GTCATATCTAAGGACCCGCGTGG

100

76.30

Chr09

35542701

35542723

crRNA_RF_2_F

TCTGTACTCCGTCTGTCGGTCGG

100

77.90

Chr09

35542848

35542870

crRNA_RF_3_R

AGAAGACTGTCAATCCCGAGTGG

100

79.60

Chr09

35550689

35550711

crRNA_RF_4_F

TGTCTGGAAAGTTTCTAACGCGG

100

70.80

Chr09

35551878

35551900

  1. Off-target score (specificity) for guide RNA sequences were calculated according to [51] against (‘Golden Delicious’ double haploid GDDH13v1.1) [34], with a score of 100% predicting low off target activity. On target activity scores (efficiency) were calculated according to [52].