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Fig. 6 | Plant Methods

Fig. 6

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

Fig. 6

adapted from Oxford Nanopore Technology (ONT). a Targeted-excision approach. The crRNAs are designed to either side of the region of interest (ROI). Both ends of the ROI must be known to be able to design the crRNAs. Indicated when the ROI is shorter than 20 kb (specific genes or genomic regions). b Single cut-walking. A single crRNA is designed at one known end of a ROI to walk towards the unknown. Indicated to target a ROI when just one side is known and for genome walking approaches of targeted regions shorter than 20 kb. c Tiling approach. Several crRNAs are designed across a ROI (which can be longer than 20 kb) in 5–10 kb overlapping pieces to get an even coverage along the region. Indicated especially when the length of the input DNA is limited

crRNA design approaches,

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