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Fig. 2 | Plant Methods

Fig. 2

From: CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants

Fig. 2

Guide RNA Cas9 digestion efficiency in PCR-amplicons linked to the apple MYB10 locus. Fragment analysis evaluating the four gRNAs designed: graphics A to D are for crRNA_RF_1 to 4, respectively. The first two peaks on A–C correspond to both fragments after Cas9 digestion. D shows a single digested fragment because the digestion occurred in the middle of the original PCR amplicon. The longest fragments correspond to undigested original PCR amplicons. Lower marker (LM) set to 1 bp and 6000 bp upper marker (UM) from the high sensitivity NGS fragment analysis kit (1–6000 bp), DNF-474, Advance Analytical

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