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Table 4 Yield and quality control of DNA extracted from six recalcitrant species using three different methods: SILEX, standard CTAB, and SMP kit quantified by NanoDrop (ND) and Qubit (Q)

From: SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

Species/Methods

Yield (ng/mg) 

Absorbance ratios

Ratio ND/Q

ND

Q

A260/A280

A260/A230

Cassava

 SILEX

556.0 ± 76.6

106.9 ± 17.4

2.04 ± 0.02 

2.13 ± 0.05 

5.3 

 CTAB

512.7 ± 61.1

37.7 ± 27.7

2.12 ± 0.02 

1.96 ± 009 

13.6 

 SMP kit

75.4 ± 9.4

85.5 ± 13.9

1.80 ± 0.09 

1.44 ± 0.10 

0.9 

Grapevine

 SILEX

442.8 ± 24.1

318.0 ± 115.2

2.07 ± 0.02 

1.93 ± 0.01 

1.4 

 CTAB

394.3 ± 18.7

21.5 ± 6.1

1.60 ± 0.05 

0.44 ± 0.03 

18.4 

 SMP kit

50.7 ± 30.2

0.0 ± 0.0

1.73 ± 0.20 

1.09 ± 0.09

Loquat

 SILEX

284.4 ± 24.9

92.7 ± 35.5

2.02 ± 0.05 

1.71 ± 0.06 

3.1 

 CTAB

112.7 ± 7.5

4.3 ± 2.1

1.58 ± 0.17 

0.57 ± 0.08 

25.9 

 SMP kit

66.7 ± 36.0

7.7 ± 6.1

1.78 ± 0.25 

1.15 ± 0.41 

8.6 

Banana

 SILEX

267.8 ± 5.1

59.2 ± 8.2

2.13 ± 0.01 

2.16 ± 0.05 

4.5 

 CTAB

202.0 ± 37.5

19.1 ± 5.6

2.20 ± 0.14 

0.78 ± 0.13 

10.6 

 SMP kit

31.4 ± 37.5

1.3 ± 2.3

1.47 ± 0.31 

0.74 ± 0.32 

23.3

Naranjillo

 SILEX

1184.1 ± 484.3

180.4 ± 60.6

2.06 ± 0.02 

1.85 ± 0.03 

6,6

 CTAB

525.4 ± 126.2

26.0 ± 7.7

1.91 ± 0.11 

0.86 ± 0.20 

20.2 

 SMP kit

105.4 ± 35.8

42.6 ± 19.8

1.82 ± 0.04 

1.72 ± 0.07 

2.5 

Strawberry

 SILEX

193.8 ± 5.1

46.4 ± 6.1

1.86 ± 0.05 

1.02 ± 0.08 

4.2 

 CTAB

405.3 ± 108.5

24.6 ± 4.0

1.77 ± 0.08 

1.14 ± 0.11 

16.5 

 SMP kit

25.9 ± 2.8

20.0 ± 14.3

1.69 ± 0.16 

0.97 ± 0.02 

1.3 

  1. Ratios of the latter are reported. Mean value and standard deviation are based on a minimum of three independent extractions