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Table 4 Yield and quality control of DNA extracted from six recalcitrant species using three different methods: SILEX, standard CTAB, and SMP kit quantified by NanoDrop (ND) and Qubit (Q)

From: SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

Species/Methods Yield (ng/mg)  Absorbance ratios Ratio ND/Q
ND Q A260/A280 A260/A230
Cassava
 SILEX 556.0 ± 76.6 106.9 ± 17.4 2.04 ± 0.02  2.13 ± 0.05  5.3 
 CTAB 512.7 ± 61.1 37.7 ± 27.7 2.12 ± 0.02  1.96 ± 009  13.6 
 SMP kit 75.4 ± 9.4 85.5 ± 13.9 1.80 ± 0.09  1.44 ± 0.10  0.9 
Grapevine
 SILEX 442.8 ± 24.1 318.0 ± 115.2 2.07 ± 0.02  1.93 ± 0.01  1.4 
 CTAB 394.3 ± 18.7 21.5 ± 6.1 1.60 ± 0.05  0.44 ± 0.03  18.4 
 SMP kit 50.7 ± 30.2 0.0 ± 0.0 1.73 ± 0.20  1.09 ± 0.09
Loquat
 SILEX 284.4 ± 24.9 92.7 ± 35.5 2.02 ± 0.05  1.71 ± 0.06  3.1 
 CTAB 112.7 ± 7.5 4.3 ± 2.1 1.58 ± 0.17  0.57 ± 0.08  25.9 
 SMP kit 66.7 ± 36.0 7.7 ± 6.1 1.78 ± 0.25  1.15 ± 0.41  8.6 
Banana
 SILEX 267.8 ± 5.1 59.2 ± 8.2 2.13 ± 0.01  2.16 ± 0.05  4.5 
 CTAB 202.0 ± 37.5 19.1 ± 5.6 2.20 ± 0.14  0.78 ± 0.13  10.6 
 SMP kit 31.4 ± 37.5 1.3 ± 2.3 1.47 ± 0.31  0.74 ± 0.32  23.3
Naranjillo
 SILEX 1184.1 ± 484.3 180.4 ± 60.6 2.06 ± 0.02  1.85 ± 0.03  6,6
 CTAB 525.4 ± 126.2 26.0 ± 7.7 1.91 ± 0.11  0.86 ± 0.20  20.2 
 SMP kit 105.4 ± 35.8 42.6 ± 19.8 1.82 ± 0.04  1.72 ± 0.07  2.5 
Strawberry
 SILEX 193.8 ± 5.1 46.4 ± 6.1 1.86 ± 0.05  1.02 ± 0.08  4.2 
 CTAB 405.3 ± 108.5 24.6 ± 4.0 1.77 ± 0.08  1.14 ± 0.11  16.5 
 SMP kit 25.9 ± 2.8 20.0 ± 14.3 1.69 ± 0.16  0.97 ± 0.02  1.3 
  1. Ratios of the latter are reported. Mean value and standard deviation are based on a minimum of three independent extractions