Fig. 7
Recognition and qualification of cells in a transverse section of a Populus trichocarpa stem. a Images of Populus trichocarpa stem cells captured by light microscopy (LM) after staining with toluidine blue and images after clearness, threshold adjustment and particle recognition, bar = 100 μm. b Graphical user interface of ImageJ software, including the main interface, ROI manager panel, and results display window. ROI list and the cellular parameters characterized from Populus trichocarpa stem cells in a. c–f Cellular parameters of Arabidopsis seed cells. Quantification of area, perimeter, and Feret’s diameter of Arabidopsis seed cells in a. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum, n = 1243 cells (c). Relative frequency distribution (percentage, %) analysis of area (d), perimeter (e), and Feret’s diameter (f) shown in c