Fig. 3

Recognition and qualification of cells in a vertical Arabidopsis root section. a Images of mCherry-labeled Arabidopsis root cells captured by Laser scanning confocal microscopy (LSCM) and images after clearness, threshold adjustment, and particle recognition, bar = 10 μm. b Graphical user interface, including the main interface, ROI (region of interest) manager panel, and the results display window of ImageJ software. The ROI list and results of the cellular parameters were identified and characterized, respectively, from Arabidopsis root cells in a. c–f Parameters of Arabidopsis root cells. Quantification of area, perimeter, and Feret’s diameter of Arabidopsis root cells in a. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum. n = 58 cells (c). Relative frequency distribution (percentage, %) analysis of area (d), perimeter (e), and Feret’s diameter (f) shown in c