Skip to main content

Table 1 Fluorescence counts and aggregation status of YFP-AtGALS1 microsomes in different incubation buffers

From: Microscale thermophoresis as a powerful tool for screening glycosyltransferases involved in cell wall biosynthesis

Buffer composition

Fluorescence counts

Aggregation observed

HEPES pH 7.0, 50 mM NaCl, 5 mM MnCl2, 1% Tx100

1247

(+)

PBS pH 7.4, 5 mM MnCl2, 0.05% Tween

479

−

MES pH 6.5, 5 mM MnCl2, 125 mM KCl, 1% Tx100

974

+

MES pH 6.5, 5 mM MnCl2, 1% Tx100

1263

−

MES pH 6.5, 5 mM MnCl2, 0.05% Tween

101

++

MES pH 6.5, 5 mM MnCl2, 0.5% Tween

174

+

MES pH 6.5, 5 mM MnCl2, 2% Tween

272

−

  1. MES buffer with 1% Triton X-100 provides highest fluorescence counts and did not produce bumpy thermograph curve as indication of protein aggregation. Tween as detergent with concentration from 0.05 to 2% did not work well with MES. Thermographs corresponding to the different conditions are shown in Additional file 1: Figure S1
Back to article page

Plant Methods

ISSN: 1746-4811