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Table 1 Fluorescence counts and aggregation status of YFP-AtGALS1 microsomes in different incubation buffers

From: Microscale thermophoresis as a powerful tool for screening glycosyltransferases involved in cell wall biosynthesis

Buffer composition Fluorescence counts Aggregation observed
HEPES pH 7.0, 50 mM NaCl, 5 mM MnCl2, 1% Tx100 1247 (+)
PBS pH 7.4, 5 mM MnCl2, 0.05% Tween 479
MES pH 6.5, 5 mM MnCl2, 125 mM KCl, 1% Tx100 974 +
MES pH 6.5, 5 mM MnCl2, 1% Tx100 1263
MES pH 6.5, 5 mM MnCl2, 0.05% Tween 101 ++
MES pH 6.5, 5 mM MnCl2, 0.5% Tween 174 +
MES pH 6.5, 5 mM MnCl2, 2% Tween 272
  1. MES buffer with 1% Triton X-100 provides highest fluorescence counts and did not produce bumpy thermograph curve as indication of protein aggregation. Tween as detergent with concentration from 0.05 to 2% did not work well with MES. Thermographs corresponding to the different conditions are shown in Additional file 1: Figure S1