Fig. 3
Substrate specificity assay of AtGALS1. Dose–response curve for the binding interaction between YFP-AtGALS1 and a UDP-Araf, or b UDP. Concentration of YFP-AtGALS1 was kept constant, while the ligand varied from 2.5 mM to 76.3 nM. Data were fitted to Kd model and confidence interval of the Kd is calculated from the variance of the fitted parameter, derived from Levenberg–Marquardt fit-algorithm. Error Bar: SD, n = 3. Values are the average ± 68% confidence