From: Optimization of isolation and transfection conditions of maize endosperm protoplasts
Source | Sum of squares | DF | Mean square | F-value | p-value | R-square |
---|---|---|---|---|---|---|
Model | 178.89 | 14 | 12.78 | 30.91 | < 0.0001 S | 0.9687 |
X1-Cellulase Conc. | 35.31 | 1 | 35.31 | 85.4 | < 0.0001 | |
X2-Pectinase Conc. | 41.06 | 1 | 41.06 | 99.32 | < 0.0001 | |
X3-Mannitol | 20.6 | 1 | 20.6 | 49.83 | < 0.0001 | |
X4-Hydrolysis time | 0.32 | 1 | 0.32 | 0.78 | 0.3924 | |
X1X2 | 0.11 | 1 | 0.11 | 0.26 | 0.6211 | |
X1X3 | 0.05 | 1 | 0.05 | 0.12 | 0.7316 | |
X1X4 | 0.3 | 1 | 0.3 | 0.73 | 0.4067 | |
X2X3 | 0.68 | 1 | 0.68 | 1.65 | 0.2203 | |
X2X4 | 2.84E−14 | 1 | 2.84E−14 | 6.87E−14 | 1 | |
X3X4 | 1.76 | 1 | 1.76 | 4.25 | 0.0584 | |
X21 | 62.27 | 1 | 62.27 | 150.61 | < 0.0001 | |
X22 | 79.95 | 1 | 79.95 | 193.38 | < 0.0001 | |
X23 | 47.89 | 1 | 47.89 | 115.82 | < 0.0001 | |
X24 | 79.1 | 1 | 79.1 | 191.32 | < 0.0001 | |
Residual | 5.79 | 14 | 0.41 | |||
Lack of Fit | 5.26 | 10 | 0.53 | 3.94 | 0.0990 NS | |
Pure error | 0.53 | 4 | 0.13 | |||
Corrected total | 184.68 | 28 |
Factor | Name | Optimal level | Experimental level range | |||
---|---|---|---|---|---|---|
Optimum | Low | Centre | High | |||
Optimization for yield of isolated maize endosperm protoplast | ||||||
X1 | Cellulase Conc. | 1 | 1 | 1.5 | 2 | |
X2 | Pectinase Conc. | 0.75 | 0.5 | 0.75 | 1 | |
X3 | Mannitol | 0.4 | 0.4 | 0.6 | 0.8 | |
X4 | Hydrolysis time | 6 | 4 | 6 | 8 |
Predicted response | Protoplast yield | Standard deviation | SE mean | 95% CI low | 95% CI high | |
---|---|---|---|---|---|---|
Optimal response | ||||||
× 106 | ||||||
2.43 | 0.643 | 0.491 | 1.38 | 3.49 |