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Table 3 Quadratic model ANOVA and optimization for protoplast yield

From: Optimization of isolation and transfection conditions of maize endosperm protoplasts

Source Sum of squares DF Mean square F-value p-value R-square
Model 178.89 14 12.78 30.91 < 0.0001 S 0.9687
X1-Cellulase Conc. 35.31 1 35.31 85.4 < 0.0001  
X2-Pectinase Conc. 41.06 1 41.06 99.32 < 0.0001  
X3-Mannitol 20.6 1 20.6 49.83 < 0.0001  
X4-Hydrolysis time 0.32 1 0.32 0.78 0.3924  
X1X2 0.11 1 0.11 0.26 0.6211  
X1X3 0.05 1 0.05 0.12 0.7316  
X1X4 0.3 1 0.3 0.73 0.4067  
X2X3 0.68 1 0.68 1.65 0.2203  
X2X4 2.84E−14 1 2.84E−14 6.87E−14 1  
X3X4 1.76 1 1.76 4.25 0.0584  
X21 62.27 1 62.27 150.61 < 0.0001  
X22 79.95 1 79.95 193.38 < 0.0001  
X23 47.89 1 47.89 115.82 < 0.0001  
X24 79.1 1 79.1 191.32 < 0.0001  
Residual 5.79 14 0.41    
Lack of Fit 5.26 10 0.53 3.94 0.0990 NS
Pure error 0.53 4 0.13    
Corrected total 184.68 28     
Factor Name Optimal level Experimental level range  
Optimum Low Centre High  
Optimization for yield of isolated maize endosperm protoplast
 X1 Cellulase Conc. 1 1 1.5 2  
 X2 Pectinase Conc. 0.75 0.5 0.75 1  
 X3 Mannitol 0.4 0.4 0.6 0.8  
 X4 Hydrolysis time 6 4 6 8  
Predicted response Protoplast yield Standard deviation SE mean 95% CI low 95% CI high  
Optimal response
  × 106      
  2.43 0.643 0.491 1.38 3.49  
  1. N Significant, NS non-significant, SE standard error, CI confidence interval