Fig. 3From: Optimization of isolation and transfection conditions of maize endosperm protoplastsApplication of endosperm protoplast system in protein immunoblotting, subcellular localization, BiFC and promoter activation analysis. a Expression of GFP protein, b subcellular localization of ZmBt1, c interaction of O2 and PBF1 proteins by BiFC assay, d positive activation of ZmBt1 promoter by ZmMYB14; mean ± SE, t test, p < 0.001 (**), n = 3Back to article page