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Table 5 Genotype detection of T1 marker-free plants generated with the “double T-DNA” vector system of pBID RT Enhanced

From: Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants

T0 events

Number of T1 plants

EPSPS+Bar+

Total number

Bar+EPSPS+GOX+

BarEPSPS+GOX+

Bar+EPSPSGOX

BarEPSPSGOX

Marker-free plant frequency (%)

pBID RT Enhanced-5

18

14

0

4

0

22

pBID RT Enhanced-16

12

0

1

0

11

/

pBID RT Enhanced-26

15

0

0

0

15

/

pBID RT Enhanced-27

18

11

2

1

4

6

pBID RT Enhanced-29

19

12

3

0

4

/

pBID RT Enhanced-31

17

15

1

1

0

6

pBID RT Enhanced-41

18

14

0

4

0

22

pBID RT Enhanced-65

17

10

0

7

0

41

Average for the marker-free selfed lines

     

19

pBID RT Enhanced-27 *ZS6

17

6

1

7

3

41

pBID RT Enhanced-29 *ZS6

23

12

2

1

8

4

pBID RT Enhanced-31 *ZS6

19

17

0

2

0

11

pBID RT Enhanced-41 *ZS6

17

8

0

9

0

53

pBID RT Enhanced-65 *ZS6

22

10

9

1

2

5

Average for the marker-free backcrossed lines

     

23

Average for all the marker-free lines

     

21

  1. T1 plants were generated by selfing and by backcrossing to WT ZS6