Table 4 Genotyping detection in T1 transformants of the mixed-strain system and the “double T-DNA” vector system of pDB1300-3300 as detected by PCR and test strips
T0 events | Number of T1 plants | |||||
|---|---|---|---|---|---|---|
HPT+Bar+ | Total number | Bar+HPT+ | Bar−HPT+ | Bar+HPT− | Bar−HPT− | Marker-free plant frequency (%) |
pCAMBIA1300/3300 (1:8)-10 | 31 | 26 | 0 | 1 | 4 | 3 |
pCAMBIA1300/3300 (1:8)-15 | 38 | 28 | 3 | 3 | 4 | 8 |
pCAMBIA1300/3300 (1:8)-24 | 13 | 7 | 6 | 0 | 0 | / |
pCAMBIA1300/3300 (1:4)-10 | 47 | 39 | 0 | 1 | 7 | 3 |
pCAMBIA1300/3300 (1:2)-1 | 43 | 37 | 0 | 0 | 6 | / |
pCAMBIA1300/3300 (1:2)-2 | 17 | 13 | 0 | 0 | 4 | / |
pCAMBIA1300/3300 (1:2)-5 | 35 | 15 | 9 | 5 | 6 | 14 |
pCAMBIA1300/3300 (1:1)-30 | 40 | 36 | 1 | 1 | 2 | 3 |
pCAMBIA1300/3300 (2:1)-30 | 21 | 18 | 0 | 0 | 3 | / |
pCAMBIA1300/3300 (8:1)-15 | 22 | 9 | 3 | 8 | 2 | 36 |
Average for the marker-free lines | 11 | |||||
pDB1300-3300-3 | 25 | 13 | 5 | 5 | 2 | 20 |
pDB1300-3300-11 | 10 | 10 | 0 | 0 | 0 | / |