Skip to main content
Fig. 6 | Plant Methods

Fig. 6

From: Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants

Fig. 6

Southern blot detection in plants from the “double T-DNA” system of pBID RT Enhanced. Bar (a) and EPSPS (b) gene copy numbers were detected in T0 transgenic B. napus plants, 1-17: T0 individual plants with the Bar+EPSPS+ genotype. Bar (c, e) and EPSPS (d, f) gene detection for identification of marker-free and herbicide resistant plants in the T1 generation, 1-12: T1 individual plants with the Bar+EPSPS− genotype, 13-24: T1 individual plants with the Bar+EPSPS+ genotype. M: DNA molecular weight marker II, DIG-labelled; WT: wild type ZS6; P: expression vector pBID RT Enhanced

Back to article page

Plant Methods

ISSN: 1746-4811