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Fig. 5 | Plant Methods

Fig. 5

From: Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants

Fig. 5

Detection of T0 transgenic plants from the “double T-DNA” system of pBID RT Enhanced. Putative T0 transformants were detected by PCR for Bar (a), EPSPS (b), and GOX (c) genes, M: DL 2000 DNA marker; 1-17: individual T0 plants; P: expression vector pBID RT Enhanced; WT: wild type ZS6. Putative T0 transgenic B. napus plants were detected with test strips for Bar (d) and EPSPS (e) protein, P: positive transgenic B. napus plant of MS8 with the Bar protein for d and GT73 with the EPSPS protein for e; WT: wild type ZS6

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Plant Methods

ISSN: 1746-4811