Fig. 4

Southern blot detection in plants. Bar (a) and HPT (b) gene copy numbers were detected in T₀ transgenic B. napus plants (lanes 1-15) from pCAMBIA1300/3300 of a variety of ratios (X:X) with the Bar⁺HPT⁺ genotype from the mixed-strain system. Bar (c) and HPT (d) gene were detected for identification of marker-free plants in the T₁ generation from the mixed-strain system, 1, 9 and 11 are T₁ progeny with the Bar⁺HPT⁻ genotype from T₀ plants as indicated in lanes 1, 9 and 11 of a, b. Bar (e) and HPT (f) gene copy numbers were detected in T₀ plants with the Bar⁺HPT⁺ genotype (lanes 1-2). Bar (e) and HPT (f) gene were detected in T₁ individual plants including Bar⁺HPT⁺ plants and marker-free Bar⁺HPT⁻ plants from T₀ plant as indicated in lanes 1 of e, f (lanes 3-7) from the “double T-DNA” system of pCAMBIA1300-3300. M: DNA molecular weight marker II, DIG-labelled; WT: wild type ZS6; P: expression vector pCAMBIA3300 for (a, c, e) and pCAMBIA1300 for (b, d, f)