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Fig. 2 | Plant Methods

Fig. 2

From: Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants

Fig. 2

Detection of T0 transformants from the mixed-strain system and “double T-DNA” system of pDB1300-3300. PCR detection was done for Bar (a) and HPT (b) genes, M: DL1000 DNA marker; 1–22: individual T0 plants from pCAMBIA1300/3300 of a variety of ratios (X:X) and plants from pDB1300-3300); P: expression vector pCAMBIA3300 for (a) and pCAMBIA1300 for (b); WT: wild type ZS6. Test strips detection was done for the Bar protein (c), 1–17: individual T0 plants from pCAMBIA1300/3300 of a variety of ratios and plants from pDB1300-3300; P: positive transgenic B. napus plant of Ms8 with the Bar protein; WT: wild type ZS6. The figure represented part of the results

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