Table 2 Primers sequences and amplification cycles for the TBP reaction
Intron region | Primer name | Primer sequence | Thermal profile |
|---|---|---|---|
1st | Fex1 | AAC TGG GCB AAR GGN CAY TAY AC | TBPTD1i |
Rex1 | ACC ATR CAY TCR TCD GCR TTY TC | ||
2nd | Fin2 | GAR AAY GCH GAY GAR TGY ATG | TBPTD2i |
Rin2 | CRA AVC CBA CCA TGA ARA ART G |
T (°C) | Time | Cycle N° | |
|---|---|---|---|
TBPTD1i | |||
94 | 3 min | ||
94 | 30 s | ||
65 | 45 s | − 0.5 °C touchdown | × 14 |
72 | 2 min | ||
94 | 30 s | ||
57 | 45 s | × 15 | |
72 | 2 min | ||
72 | 30 min | ||
TBPTD2i | |||
94 | 3 min | ||
94 | 30 s | ||
65 | 45 s | −0.7 °C touchdown | × 14 |
72 | 2 min | ||
94 | 30 s | ||
55 | 45 s | × 15 | |
72 | 2 min | ||
72 | 30 min | ||