Table 3 RNA concentration and purity of the RNA obtained from different RNA extraction methods for different B. napus and B. oleracea tissue types

E.Z.N.A. Plant RNA kit

Green stage seed coat (GSSC)

32.66

1.67

0.30

TRIzol Reagent

Green stage seed coat (GSSC)

1.57

0.73

0.43

Picopure RNA Isolation kit

Green stage seed coat (GSSC)

113.55

2.03

1.42

E.Z.N.A. Plant RNA kit

Green stage endosperm (GSEnd)

7.79

1.39

0.14

Picopure RNA Isolation kit

Green stage endosperm (GSEnd)

54.64

1.80

0.58

Picopure RNA Isolation kit

Green stage endosperm (GSEnd) + PVPP

147.57

2.11

1.74

Hot borate

Mature stage seed coat (MSSC)

24.15

1.62

0.47

Picopure RNA Isolation kit

Mature stage seed coat (MSSC)

53.86

2.11

0.53

Picopure RNA Isolation kit

Mature stage seed coat (MSSC) + PVPP

58.27

2.02

1.59

E.Z.N.A. Plant RNA kit

Heart stage embryo (HSE)

16.16

2.30

0.10

Picopure RNA Isolation kit

Heart stage embryo (HSE)

104.10

2.07

1.97

E.Z.N.A. Plant RNA kit

Torpedo stage embryo (TSE)

91.20

2.15

2.14

Picopure RNA Isolation kit

Torpedo stage embryo (TSE)

218.14

2.13

2.09