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Table 2 Range of RNA concentration, A260/280 and A260/230 ratios for B. napus and B. oleracea tissue types extracted with the E.Z.N.A Plant RNA kit, n=15

From: Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseed Brassica seed tissues during seed development

Tissue

RNA concentration (ng/µl)

A260/280

A260/230

Ovules

158.8–382.7

2.10–2.19

1.80–2.20

Ovary wall

380.2–827.5

2.17–2.21

1.88–2.21

Green stage embryo (GSE)

749.8–1702.2

2.19–2.22

1.70–2.24

Mature stage embryo (MSE)

127.3–639.9

2.15–2.21

1.94–2.26

Heart stage endosperm (HSEnd)

108.8–637.9

2.17–2.20

1.96–2.20

Torpedo stage endosperm (TSEnd)

102.0–243.4

2.18–2.21

1.80–2.16

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