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Table 1 Biochemical characterization of Arabidopsis GTRs for different glucosinolate substrates using the cotton cell line system. Uptake assays for three independent clones (100 mg) were performed with glucosinolate concentration (5–800 µM) for 1 h. Data represents mean ± SE

From: A cell suspension based uptake method to study high affinity glucosinolate transporters

Substrate

Transporter

Km (µM)

Vmax (nanomoles/g/min)

Gluconapin

AtGTR1

279.1 ± 109

3.4 ± 0.6

Gluconapin

AtGTR2

153.3 ± 59

2.9 ± 0.4

Sinigrin

AtGTR1

117.9 ± 44

0.5 ± 0.07

Sinigrin

AtGTR2

436.7 ± 154

4.6 ± 1.1

Sinalbin

AtGTR1

104.5 ± 56

0.5 ± 0.09

Sinalbin

AtGTR2

166.7 ± 40

1.2 ± 0.1