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Fig. 4 | Plant Methods

Fig. 4

From: A cell suspension based uptake method to study high affinity glucosinolate transporters

Fig. 4

Standardization of transport uptake assays using AtGTR expressing CCL-1. a Effect of presence of nitrate salts in millimolar range in uptake buffer on uptake efficiency using ATGTR2 expressing CCL-1. The uptake was performed at 10 µM and 100 µM of sinigrin in uptake buffer for 1 h. b Effect of pH of uptake buffer on uptake efficiency using AtGTR2 expressing cells. c Time based uptake of sinigrin through 0–24 h duration. Uptake assays for three independent clones were performed with sinigrin at 100 µM for 1 h, unless indicated. Data represents mean ± SE (n = 3), and student’s t-test, two tail distribution was performed with a threshold p value of 0.05

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