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Fig. 2 | Plant Methods

Fig. 2

From: A cell suspension based uptake method to study high affinity glucosinolate transporters

Fig. 2

Generation and validation of GTR over-expressing CCL-1. a T-DNA map representing GTR over-expression construct. b Starting from left, untransformed cells on Basta selection media, GTR transformant clones growing through dead untransformed cells on selection media, GTR transformant clone maintained on selection media; (c) and (d) qRT-PCR analysis showing relative fold expression of AtGTR1 and AtGTR2 in CCL-1 transformants, respectively (w.r.t. cotton Ubiquitin set at 100). Six clones each of AtGTR1 and AtGTR2 over-expressing CCL-1 are presented along with vector controls (VC1 and VC2)

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