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Fig. 4 | Plant Methods

Fig. 4

From: Ice accommodation in plant tissues pinpointed by cryo-microscopy in reflected-polarised-light

Fig. 4

Experimental setup for CMrpl: The microscope, excluding the camera unit (1), was placed inside the fully temperature-controlled environment of a laboratory chest freezer (2). A customised transparent acrylic glass lid (3) substituted the standard lid. The lid was equipped with insulated gloves (4) for manipulation inside the temperature-controlled environment. For the microscopic investigations, leaves were clamped into the sample holder (5) or buds with approx. 1 cm long shoots placed on top of it. Orientation of the polarised light was controlled by the polariser (6) and the analyser (7). Illumination was provided by an external cold light source, which was connected to the microscope with fiber optics (8)

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