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Table 1 Overview of analytical methods for strigolactones

From: Analytical methods in strigolactone research

Analytical technique Column Mobile phase composition Analysis time Matrix Extraction solvent Purification Strigolactone References
LC-ESI-MS/MS ODS (C18), Mightysil RP-18
(2 × 250 mm, 5 µm)
A—H2O
B—MeOH
34–60 min Tissue EtOAc LLE 5-Deoxystrigol
7-Oxoorobanchyl acetate
7α-Hydroxyorobanchyl acetate
Didehydro-orobanchol
Fabacyl acetate
Methyl zealactonoate
Orobanchol
Orobanchyl acetate
Solanacol
Sorgolactone
Sorgomol
Strigol
Strigyl acetate
[5, 15, 18, 30, 32, 40, 41, 43,44,45, 48, 49]
Exudates Adsorption on charcoal and elution with acetone; LLE with EtOAc
L-Column2 ODS
(2.1 × 50 mm, 2.0 μm)
A—H2O
B—MeOH
22 min Exudates Adsorption on charcoal and elution with acetone; LLE with EtOAc 5-Deoxystrigol
Orobanchol
Orobanchyl acetate
[25]
COSMOSIL 2.5C18-MS-II (100 × 2.0 mm, 2.5 µm) A—H2O
B—MeOH
20 min Exudates EtOAc CC (silica) 4-Deoxyorobanchol
5-Deoxystrigol
Carlactone
Carlactonoic acid
Heliolactone
[16, 52]
ACE Excel 1.7
C18 (100 mm × 2.1 mm, 1.7 μm)
A—0.1% FA/H2O
B—0.1% FA/ACN
10.5 min Exudates Adsorption on C18 and elution with acetone 5-Deoxystrigol
7-Oxoorobanchyl acetate
Fabacyl acetate
Orobanchol
Orobanchyl acetate
Solanacol
Strigol
[29]
Tissue EtOAC
ACQUITY BEH C18 (100 × 2.1 mm, 1.7 mm) A—0.1% FA/H2O
B—ACN
12 min Exudates Adsorption on C18 and elution with acetone Zealactones 1-5 [28]
A—H2O
B—ACN
19 min Tissue EtOAc CC (silica) Fabacyl acetate
Orobanchol
Orobanchyl acetate
[35]
A—H2O
B—MeOH
9 min Exudates Adsorption on C18 and elution with acetone 5-Deoxystrigol
7-Oxoorobanchyl acetate
Fabacyl acetate
Orobanchol
Orobanchyl acetate
Solanacol
Strigol
[33]
ACCLAIM 120C18
(2.1 mm × 250 mm, 5 μm)
A—0.1% FA/H2O
B—0.1% FA/ACN
40 min Exudates EtOAc C18 SPE 5-Deoxystrigol
Fabacyl acetate
Orobanchyl acetate
Orobanchol
Solanacol
[23]
Tissue EtOAc CC (silica)
Kinetex C18 (2.1 × 150 mm, 2.6 μm) A—0.1% AcA/H2O
B—0.1% AcA/ACN
23 min Tissue EtOAc CC (silica) 5-Deoxystrigol [34]
GC-MS DB-5 (4 m × 0.25 mm) He 27 min Exudates Adsorption on XAD-4 and elution with EtOAc; CC on Sephadex LH-20 Methyl zealactonoate
Orobanchol
[27, 47, 48]
Tissue EtOAc
  1. AcA acetic acid, FA formic acid, LLE liquid–liquid extraction, CC column chromatography, SPE solid phase extraction
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