Analytical methods in strigolactone research

Halouzka, Rostislav; Zeljković, Sanja Ćavar; Klejdus, Bořivoj; Tarkowski, Petr

doi:10.1186/s13007-020-00616-2

Plant Methods

Table 1 Overview of analytical methods for strigolactones

From: Analytical methods in strigolactone research

Analytical technique	Column	Mobile phase composition	Analysis time	Matrix	Extraction solvent	Purification	Strigolactone	References
LC-ESI-MS/MS	ODS (C18), Mightysil RP-18 (2 × 250 mm, 5 µm)	A—H₂O B—MeOH	34–60 min	Tissue	EtOAc	LLE	5-Deoxystrigol 7-Oxoorobanchyl acetate 7α-Hydroxyorobanchyl acetate Didehydro-orobanchol Fabacyl acetate Methyl zealactonoate Orobanchol Orobanchyl acetate Solanacol Sorgolactone Sorgomol Strigol Strigyl acetate	[5, 15, 18, 30, 32, 40, 41, 43,44,45, 48, 49]
	ODS (C18), Mightysil RP-18 (2 × 250 mm, 5 µm)	A—H₂O B—MeOH	34–60 min	Exudates	–	Adsorption on charcoal and elution with acetone; LLE with EtOAc		[5, 15, 18, 30, 32, 40, 41, 43,44,45, 48, 49]
	L-Column2 ODS (2.1 × 50 mm, 2.0 μm)	A—H₂O B—MeOH	22 min	Exudates	–	Adsorption on charcoal and elution with acetone; LLE with EtOAc	5-Deoxystrigol Orobanchol Orobanchyl acetate	[25]
	COSMOSIL 2.5C18-MS-II (100 × 2.0 mm, 2.5 µm)	A—H₂O B—MeOH	20 min	Exudates	EtOAc	CC (silica)	4-Deoxyorobanchol 5-Deoxystrigol Carlactone Carlactonoic acid Heliolactone	[16, 52]
	ACE Excel 1.7 C18 (100 mm × 2.1 mm, 1.7 μm)	A—0.1% FA/H₂O B—0.1% FA/ACN	10.5 min	Exudates	–	Adsorption on C18 and elution with acetone	5-Deoxystrigol 7-Oxoorobanchyl acetate Fabacyl acetate Orobanchol Orobanchyl acetate Solanacol Strigol	[29]
	ACE Excel 1.7 C18 (100 mm × 2.1 mm, 1.7 μm)	A—0.1% FA/H₂O B—0.1% FA/ACN	10.5 min	Tissue	EtOAC	Adsorption on C18 and elution with acetone		[29]
	ACQUITY BEH C18 (100 × 2.1 mm, 1.7 mm)	A—0.1% FA/H₂O B—ACN	12 min	Exudates	–	Adsorption on C18 and elution with acetone	Zealactones 1-5	[28]
		A—H₂O B—ACN	19 min	Tissue	EtOAc	CC (silica)	Fabacyl acetate Orobanchol Orobanchyl acetate	[35]
		A—H₂O B—MeOH	9 min	Exudates	–	Adsorption on C18 and elution with acetone	5-Deoxystrigol 7-Oxoorobanchyl acetate Fabacyl acetate Orobanchol Orobanchyl acetate Solanacol Strigol	[33]
	ACCLAIM 120C18 (2.1 mm × 250 mm, 5 μm)	A—0.1% FA/H₂O B—0.1% FA/ACN	40 min	Exudates	EtOAc	C18 SPE	5-Deoxystrigol Fabacyl acetate Orobanchyl acetate Orobanchol Solanacol	[23]
	ACCLAIM 120C18 (2.1 mm × 250 mm, 5 μm)	A—0.1% FA/H₂O B—0.1% FA/ACN	40 min	Tissue	EtOAc	CC (silica)		[23]
	Kinetex C18 (2.1 × 150 mm, 2.6 μm)	A—0.1% AcA/H₂O B—0.1% AcA/ACN	23 min	Tissue	EtOAc	CC (silica)	5-Deoxystrigol	[34]
GC-MS	DB-5 (4 m × 0.25 mm)	He	27 min	Exudates	–	Adsorption on XAD-4 and elution with EtOAc; CC on Sephadex LH-20	Methyl zealactonoate Orobanchol	[27, 47, 48]
GC-MS	DB-5 (4 m × 0.25 mm)	He	27 min	Tissue	EtOAc		Methyl zealactonoate Orobanchol	[27, 47, 48]

AcA acetic acid, FA formic acid, LLE liquid–liquid extraction, CC column chromatography, SPE solid phase extraction

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ISSN: 1746-4811

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