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Table 3 Comparison of the labor input between traditional method and simplified AIO-seq for 96 RIL samples

From: All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing

Step Traditional pipelinea Simplified AIO-seq pipeline
Time needed (day) Hands-on time (hour) Time needed (day) Hands-on time (hour)
Library preparationb 1.5 8 0.5 5
Size profile analysis 0.5 2 0 0
Size selection 0.5 6 0.1 0.2
qPCR of library 0.2 5 0.1 0.2
Total 2.7 21 0.7 5.4
  1. aTraditional pipeline is started from mechanical or enzymatic fragmentation
  2. bLibrary preparation includes steps from genomic DNA to the finish of the PCR