Skip to main content

Table 3 Comparison of the labor input between traditional method and simplified AIO-seq for 96 RIL samples

From: All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing

Step

Traditional pipelinea

Simplified AIO-seq pipeline

Time needed (day)

Hands-on time (hour)

Time needed (day)

Hands-on time (hour)

Library preparationb

1.5

8

0.5

5

Size profile analysis

0.5

2

0

0

Size selection

0.5

6

0.1

0.2

qPCR of library

0.2

5

0.1

0.2

Total

2.7

21

0.7

5.4

  1. aTraditional pipeline is started from mechanical or enzymatic fragmentation
  2. bLibrary preparation includes steps from genomic DNA to the finish of the PCR