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Table 1 Library pooling of 14 rice samples using AIO-seq

From: All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing

Group No. Sample ID Con. of lib (ng/μL)a Ratio of 420–520 bp (%)b Target region concentration (TRC, ng/μL) Proportion of expected data yield in groupc Mass of mixed target region (ng)c Vol. for mixing (μL)d Final data yield (Gb)
A 1 21.00 11 2.31 1/7 20.0 8.66 6.38
2 25.60 13 3.33 1/7 20.0 6.01 6.02
3 19.60 13 2.55 1/7 20.0 7.85 7.20
4 20.40 12 2.45 1/7 20.0 8.17 6.97
5 24.80 13 3.22 1/7 20.0 6.20 7.03
6 22.20 12 2.66 1/7 20.0 7.51 6.50
7 24.20 12 2.90 1/7 20.0 6.89 6.74
B 1 20.20 10 2.02 1/7 20.0 9.90 5.79
2 25.80 13 3.35 1/7 20.0 5.96 5.88
3 22.00 12 2.64 1/7 20.0 7.58 6.44
4 24.60 12 2.95 1/7 20.0 6.78 5.95
5 21.40 13 2.78 1/7 20.0 7.19 5.46
6 24.60 13 3.20 1/7 20.0 6.25 5.96
7 26.20 13 3.41 1/7 20.0 5.87 5.74
  1. aCon. of lib means the initial concentration of library for each individual assayed by Qubit™ 4.0 Fluorometer
  2. bAnalyzed by Agilent 2100 Bioanalyzer, indicates the ratio of the fragment between 420 and 520 bp, which will be size selected for sequencing
  3. cFor each group, seven samples were processed together and each sample was expected to have an equal yield of data, thus the proportion of expected data yielded for each sample was 1/7, and we mixed equal amounts of the target regions (20.0 ng) for each
  4. dVol. for mixing represents the volume of each library that needed to be pooled. Both the “Mass of mixed target region” and “Vol. for mixing” could be proportionately scaled up or down