Fig. 1

A schematic plan describing the multiplex RNAscope-ISH assay to visualize the spatio-temoral expression of more than one target RNA species in a tissues section. Fixed plant tissue section with preserved RNA in each cell (A). RNA species can be targeted or non-targeted. Binding of target ZZ probe pair (B), PreAMPs (C), AMPs and fluorescence probes (D) in a cell containing the target RNA species. Inability of ZZ probe pair, PreAMP, AMP and fluorophore binding (a, b, c) in cells containing non-targeted RNA species only. Visualization of probe fluorescence in the tissue section in spatio-temporal manner for targeted RNA species (E)