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Fig. 4 | Plant Methods

Fig. 4

From: High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis

Fig. 4

Examples of leaf lesion segmentation using the LiMu program. Images of leaves with a even and b uneven tissue clearing, acquired post-staining with trypan blue dye. c, d Labelled lesion segments from leaf images a and b. Uneven or incomplete clearing (b) can result in subsequent detection of false positive lesions (d) in poorly cleared leaf parts

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Plant Methods

ISSN: 1746-4811