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Fig. 10 | Plant Methods

Fig. 10

From: High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis

Fig. 10

Comparison of results obtained in LiMu program, IMAGEJ and manual assessment of morphometric leaf parameters. Images used for comparisons were (I) positive control images (N = 10) and (III) randomly chosen images from the experimental dataset (N = 10). Morphometric parameters such as A leaf area and B lesion area, measured as pixels (px), and C leaf damage were compared (II, IV). Leaf damage was calculated as \(Damage = \left( {\frac{lesion area}{leaf area}} \right) \times 100\). Dashed horizontal line represents the overall mean across damage levels. A nonparametric Friedman′s test was used, followed by Dunn′s post hoc test. Significant differences (p ≤ 0.05) identified in the post hoc test are indicated by different lower-case letters (a, b) (Additional file 7)

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Plant Methods

ISSN: 1746-4811