Table 3 Deparaffinization and hydration schedules for preparing slides to staining
From: An optimized histological proceeding to study the female gametophyte development in grapevine
Deparaffinization | ||
|---|---|---|
Solvent | Change | Time |
Xylene | 1 | 30′ |
Xylene | 2 | 5′ |
Hydration | ||
|---|---|---|
Ethanol concentration | Change | Time |
Absolute | 1 | 5′ |
Absolute | 2 | 5′ |
90% | 1 | 5′ |
70% | 1 | 5′ |
50% | 1 | 5′ |