Table 2 Dehydration and clearing schedules within the histological preparation process of grapevine ovaries at pre-fruitset stages
From: An optimized histological proceeding to study the female gametophyte development in grapevine
Dehydration steps | ||
|---|---|---|
Histoalcohol grade | Change | Time |
50% | 1 | 15′ |
50% | 2 | 30′ |
70% | 1 | 15′ |
70% | 2 | 30′ |
70% | 3 | 30′ |
95% | 1 | 15′ |
95% | 2 | 30′ |
99% | 1 | 30′ |
Clearing steps | ||
|---|---|---|
Solvent | Change | Time |
Xylene | 1 | 15′ |
Xylene | 1 | 30′ |