Fig. 6
From: An optimized histological proceeding to study the female gametophyte development in grapevine
Transversal sections of Sangiovese ovules at stage E-L 26 (flowering). A Sangiovese ovary at 100Ă— with two locules and four ovules with exfoliated integuments (they can partly be the result of microtome damaging). B Rare ovary presenting three locules with two ovules each at 50Ă—. C Exfoliated integuments partly due to microtome damaging, embryo sac with central cell at 400Ă—. D Egg cell and two synergids at 400Ă—. E, F Embryo sac with initiation of the endosperm at 400Ă— (E) and endosperm nuclei in detail at 1000Ă— (F). G embryo sac with an already visible zygote at 400Ă—. H one of the first transversal sections of a Sangiovese ovule at 400Ă— with an unusual shape of the inner integument. I section of the middle part of the same ovule shown in H where the fertilization process is occurring: zygote just after fertilization by one sperm cell, two dark synergids together and a large central cell with two nuclei including nucleus of the central cell (2n) and the nucleus of the second sperm cell (n), 400Ă—. J Ovule at 400Ă— with an already visible zygote and synergids (zoom in the upper right corner). K Embryo sac after fertilization where the initiation of the nuclear endosperm with several micronuclei (black dotted area, zoom in the left upper corner) and two dying synergids (red dotted area, zoom in the left bottom corner) are visible, 400Ă—. The upper synergid is at the beginning of the cell death process while the one in the bottom is already dead. L Embryo sac with dead cells in the center (*), 400Ă—. cc: central cell, ccn: central cell nucleus, ec: egg cell, en: endosperm, ii: inner integument, Nu: nucellus, oi: outer integument, s: synergids, scn: sperm cell nucleus, z: zygote