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Fig. 3 | Plant Methods

Fig. 3

From: An optimized histological proceeding to study the female gametophyte development in grapevine

Fig. 3

Longitudinal sections of Sangiovese ovules at stage E-L 17. A Anatropous ovules at 50Ă—. B Ovule with megaspore at 400Ă— in the stage of two nuclei after first mitosis. C Detailed megaspore in B at 1000Ă—. D Megagametogenesis during the second mitosis, in the stage after anaphase and near telophase (red box), at 1000Ă— with the ovule overview in the right upper corner of the image. E Ovule with well-developed nucellus around embryo sac, inner and outer integuments. F Well-developed nucellus in the ovule. ch: chalaza, f: funiculus, ii: inner integument, L: locule, m: micropyle, ms: megaspore, nc: nucellar cap, Nu: nucellus, oi: outer integument, ov: ovule

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