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Table 3 geNorm M and stability values (SV) of the eight candidate reference genes obtained by geNorm, NormFinder, BestKeeper, RefFinder algorithm and delta Ct method

From: Identification of stable reference genes for qPCR studies in common wheat (Triticum aestivum L.) seedlings under short-term drought stress

Rank

geNorm

NormFinder

BestKeeper

RefFinder

Delta Ct

Gene

geNorm M

Gene

SV

Gene

SV

Gene

SV

Gene

SV

1

CJ705892

0.554

CJ705892

0.072

ACT

0.498

ACT

1.00

ACT

0.87

2

ACT

0.573

ACT

0.084

CJ705892

0.526

UBI

1.86

UBI

0.89

3

UBI

0.596

UBI

0.131

UBI

0.564

CJ705892

2.71

CJ705892

0.89

4

GAPDH

0.676

TUB

0.139

TUB

0.732

GAPDH

4.43

GAPDH

0.99

5

TUB

0.729

BJ254354

0.152

CA644093

0.761

TUB

4.95

TUB

1.03

6

BJ254354

0.771

GAPDH

0.153

GAPDH

0.842

BJ254354

5.96

BJ254354

1.04

7

CA644093

0.839

CA644093

0.216

BJ254354

0.851

CA644093

6.44

CA644093

1.14

8

CA728440

0.975

CA728440

0.300

CA728440

1.032

CA728440

8.00

CA728440

1.46