Table 1 Primers sequences and amplicons characteristics of candidate RGs
Gene name | GenBank accession number | Primer sequence (5′ → 3′) | Amplicon length (bp) | Reference |
|---|---|---|---|---|
Triticum aestivum alpha-tubulin mRNA (TUB) | U76558 | F: CCCTGAGGTTTGATGGTGCT | 156 | Rampino et al. [40] |
R: TGGTGATCTCAGCAACGGAC | ||||
Triticum aestivum mRNA for actin (ACT) | AB181991 | F: GGAGAAGCTCGCTTACGTG | 136 | Wei et al. [43] |
R: GGGCACCTGAACCTTTCTGA | ||||
Triticum aestivum glyceraldehyde-3-phosphate dehydrogenase (GAPC) mRNA (GAPDH) | EF592180 | F: AACGACCCCTTCATCACCAC | 150 | Wei et al. [43] |
R: GTTCCTGCAGCCAAACACAG | ||||
Triticum aestivum ubiquitin (WUB1) mRNA (UBI) | AY297059 | F: GGAGTCCACCCTTCACTTGG | 130 | Li et al. [41] |
R: GACACAGGCACCATTCGAG | ||||
Wheat translation elongation factor 1 alpha-subunit (TEF1) mRNA (TEF1) | M90077.1 | F: AGGCTGACTGTGCTGTTCTC | 106 | Liu et al. [42] |
R: AGAGTGAAAGCAAGGA | ||||
EST BJ254354 | BJ254354 | F: TGTTGAGGAGACAGTTGCCC | 101 | This study |
R: GTTTGTCGGGCAAfTGCAGAG | ||||
EST wpa1c.pk012.d13 | CA596223 | F: AGAACTTGGCGTACAGGCTC | 109 | This study |
R: GGCAGAGACTCGTACATCGG | ||||
EST wdi1c.pk002.n12 | CA728440 | F: CCCATCCAGCTCACACTGAC | 134 | This study |
R: CGTGTCCGGCTTAAAACGAG | ||||
EST CJ705892 | CJ705892 | F: GCCTCAGTGGTAGGAGCATT | 116 | This study |
R: TTCAGCAAATGCGGTGGTTG | ||||
EST wre1n.pk0067.d7 | CA644093 | F: CAGTCTGCACTGTGGCACTA | 113 | This study |
R: CCAGCCGCCTAAACTTCTGA |